You'll need to configure the new sku grid account how you want it for this 2nd eBay account - see eBay guide -

You'd also need to set up whichever method you want to use to help get your items updated (i.e. ecom syndicator, file exchange, etc) -

Then if you intend on using Sku Fetch & Wise Lister for the 2nd account, you'd log into sku fetch using the new sku grid credentials and configure your settings and you'd create a new wise lister account with another email address.  Follow all steps to set up and connect the 2 -

So in short, you'd be doing everything you did for your first sku grid account, but for the 2nd one now.